Such vehicles as the Aston Martin DB5 from Goldfinger and the BMW 750i from Tomorrow Never Dies will be on show at the National Motor Museum at Beaulieu in Hampshire.
On public display from January 18 until the end of the year, the cars also include the Lotus Esprit S1 from The Spy Who Loved Me.
The exhibition will also show off various other devices used in the 007 movies, including the cello case ski navigated by Timothy Dalton when he played Bond in The Living Daylights.
The public will also see a Bede Acrostar mini jet flown in the 007 film Octopussy.
The museum has a collection of more 250 vehicles telling the story of motoring on Britain’s roads.
The collection includes racing cars, modern rally cars and the latest Formula 1 machines as well as world land speed record breakers and family cars from every motoring era.